anti rab18 antibody (Santa Cruz Biotechnology)
Structured Review

Anti Rab18 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rab18+antibody/ASF1B+Antibody/pm40565221-106-35-37
Average 93 stars, based on 7 article reviews
Images
1) Product Images from "The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP."
Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
Journal: International journal of molecular sciences
doi: 10.3390/ijms26125758
Figure Legend Snippet: Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- cGAMP/Rab18. (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-
Techniques Used: Western Blot
Figure Legend Snippet: Figure 3. MMP3 is a potential transcriptional target of the 2′3′-cGAMP/Rab18/MAPK/FosB2 signaling axis. (A) Representative immunofluorescent (IF) images of indicated MDA-MB-231 cells with DAPI and phalloidin staining. Where indicated, cells were treated with 2.5 µg/mL 2′3′-cGAMP for 24 h. (B) Representative IF images of indicated HeLa cells. Where indicated, HeLa cells were treated with 2.5 µg/mL 2′3′-cGAMP alone, PD-0325901 (1 µM) alone, or in combination for 24 h. (C,D) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for either 18 h (C) or 36 h (D). Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (E) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP alone or in combination with SCH772984 (2 µM) for 12 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (F) RT-PCR analysis of mRNA expression of indicated MMPs from indicated MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for 18 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test).
Techniques Used: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing
Related Articles
Western Blot:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Staining:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Reverse Transcription Polymerase Chain Reaction:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Expressing:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Cell Culture:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Phospho-proteomics:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Labeling:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Migration:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Activation Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Binding Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Reverse Transcription:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). SYBR Green Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Protease Inhibitor:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Purification:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Enzyme-linked Immunosorbent Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). In Vitro:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Activity Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Generated:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Derivative Assay:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Plasmid Preparation:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Control:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Stable Transfection:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Infection:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Luciferase:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Injection:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). Imaging:Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP. Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B). |
