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anti rab18 antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti rab18 antibody
    Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- <t>cGAMP/Rab18.</t> (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-
    Anti Rab18 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rab18+antibody/ASF1B+Antibody/pm40565221-106-35-37
    Average 93 stars, based on 7 article reviews
    anti rab18 antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP."

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms26125758

    Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- cGAMP/Rab18. (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-
    Figure Legend Snippet: Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- cGAMP/Rab18. (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-

    Techniques Used: Western Blot

    Figure 3. MMP3 is a potential transcriptional target of the 2′3′-cGAMP/Rab18/MAPK/FosB2 signaling axis. (A) Representative immunofluorescent (IF) images of indicated MDA-MB-231 cells with DAPI and phalloidin staining. Where indicated, cells were treated with 2.5 µg/mL 2′3′-cGAMP for 24 h. (B) Representative IF images of indicated HeLa cells. Where indicated, HeLa cells were treated with 2.5 µg/mL 2′3′-cGAMP alone, PD-0325901 (1 µM) alone, or in combination for 24 h. (C,D) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for either 18 h (C) or 36 h (D). Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (E) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP alone or in combination with SCH772984 (2 µM) for 12 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (F) RT-PCR analysis of mRNA expression of indicated MMPs from indicated MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for 18 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test).
    Figure Legend Snippet: Figure 3. MMP3 is a potential transcriptional target of the 2′3′-cGAMP/Rab18/MAPK/FosB2 signaling axis. (A) Representative immunofluorescent (IF) images of indicated MDA-MB-231 cells with DAPI and phalloidin staining. Where indicated, cells were treated with 2.5 µg/mL 2′3′-cGAMP for 24 h. (B) Representative IF images of indicated HeLa cells. Where indicated, HeLa cells were treated with 2.5 µg/mL 2′3′-cGAMP alone, PD-0325901 (1 µM) alone, or in combination for 24 h. (C,D) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for either 18 h (C) or 36 h (D). Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (E) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP alone or in combination with SCH772984 (2 µM) for 12 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (F) RT-PCR analysis of mRNA expression of indicated MMPs from indicated MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for 18 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test).

    Techniques Used: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

    Related Articles

    Western Blot:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Staining:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Expressing:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Cell Culture:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Phospho-proteomics:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Labeling:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Migration:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Activation Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Binding Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Reverse Transcription:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    SYBR Green Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Protease Inhibitor:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Purification:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    In Vitro:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Activity Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Generated:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Derivative Assay:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Plasmid Preparation:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Control:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Stable Transfection:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Infection:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Luciferase:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Injection:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).

    Imaging:

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2′3′-cGAMP
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.
    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Article Title: 2′3′-cGAMP interactome identifies 2′3′-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity
    Article Snippet: The Rneasy Mini Kit was purchased from QIAGEN (catalog no. 74106), iScript Reverse Transcription Supermix for qRT-PCR was from Bio-Rad (catalog no. 170-8891), Taq universal SYBR green supermix (Bio-Rad, catalog no. 172-5124), protease inhibitor cocktail (Bimake, catalog no. B14012), and phosphatase inhibitor cocktails A and B (Bimake, catalog no. B15001-A /B15001-B).



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    Image Search Results


    (A) The differentially expressed proteins in the LD proteome analyzed by volcano plots. LD-associated proteins were annotated. (B) The interaction network of LD-associated proteins with upregulated expression in the LD proteome from skeletal muscle of athletes compared with T2DM patients using STRING interaction network. TAG mobilization related proteins such as HSL (LIPE), ATGL (PNPLA2), ACSL3, and ACSL4 were upregulated. (C) Silver staining of proteins was performed to verify consistent sample loading across all LD samples. LD-associated protein ATGL, ADRP, pHSL, 17βHSD11, and RAB18 levels from athletes and T2DM patients were validated by immunoblotting. Lysosomal protein LAMP1 and mitochondrial protein VDAC and TIM23 were validated by immunoblotting using the corresponding antibody. (D) Silver staining of proteins was performed to verify consistent sample loading across all tissue samples. LD-associated protein ATGL and 17βHSD11, lysosomal protein LAMP1 and mitochondrial protein VDAC were validated by immunoblotting using the corresponding antibody. (E) The relative protein level of ATGL in LDs (a) and tissues (b). Grayscale analysis was conducted utilizing Image J software.

    Journal: bioRxiv

    Article Title: Lipid Droplet Proteome Reveals that Associated ATGL and Anchored Mitochondria Lead to Higher Skeletal Muscle Insulin Sensitivity in Endurance Athletes than Type 2 Diabetes Mellitus

    doi: 10.1101/2025.07.15.665019

    Figure Lengend Snippet: (A) The differentially expressed proteins in the LD proteome analyzed by volcano plots. LD-associated proteins were annotated. (B) The interaction network of LD-associated proteins with upregulated expression in the LD proteome from skeletal muscle of athletes compared with T2DM patients using STRING interaction network. TAG mobilization related proteins such as HSL (LIPE), ATGL (PNPLA2), ACSL3, and ACSL4 were upregulated. (C) Silver staining of proteins was performed to verify consistent sample loading across all LD samples. LD-associated protein ATGL, ADRP, pHSL, 17βHSD11, and RAB18 levels from athletes and T2DM patients were validated by immunoblotting. Lysosomal protein LAMP1 and mitochondrial protein VDAC and TIM23 were validated by immunoblotting using the corresponding antibody. (D) Silver staining of proteins was performed to verify consistent sample loading across all tissue samples. LD-associated protein ATGL and 17βHSD11, lysosomal protein LAMP1 and mitochondrial protein VDAC were validated by immunoblotting using the corresponding antibody. (E) The relative protein level of ATGL in LDs (a) and tissues (b). Grayscale analysis was conducted utilizing Image J software.

    Article Snippet: Antibodies against ADRP (Cat#A6276), CGI58 (Cat#A6801), Mettl7B (Cat#A7200), RAB18 (Cat#A2812), and GM130 (Cat#A5344) were purchased from Abclonal.

    Techniques: Expressing, Silver Staining, Western Blot, Software

    Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- cGAMP/Rab18. (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-

    Journal: International journal of molecular sciences

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.

    doi: 10.3390/ijms26125758

    Figure Lengend Snippet: Figure 1. Phospho-profiling identifies MAPK as a critical kinase signaling downstream of 2′3′- cGAMP/Rab18. (A) Immunoblot (IB) analysis of whole-cell lysates (WCL) from the indicated MDA-MB-

    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Techniques: Western Blot

    Figure 3. MMP3 is a potential transcriptional target of the 2′3′-cGAMP/Rab18/MAPK/FosB2 signaling axis. (A) Representative immunofluorescent (IF) images of indicated MDA-MB-231 cells with DAPI and phalloidin staining. Where indicated, cells were treated with 2.5 µg/mL 2′3′-cGAMP for 24 h. (B) Representative IF images of indicated HeLa cells. Where indicated, HeLa cells were treated with 2.5 µg/mL 2′3′-cGAMP alone, PD-0325901 (1 µM) alone, or in combination for 24 h. (C,D) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for either 18 h (C) or 36 h (D). Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (E) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP alone or in combination with SCH772984 (2 µM) for 12 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (F) RT-PCR analysis of mRNA expression of indicated MMPs from indicated MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for 18 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test).

    Journal: International journal of molecular sciences

    Article Title: The Rab18/Ras/ERK/FosB/MMP3 Signaling Pathway Mediates Cell Migration Regulation by 2'3'-cGAMP.

    doi: 10.3390/ijms26125758

    Figure Lengend Snippet: Figure 3. MMP3 is a potential transcriptional target of the 2′3′-cGAMP/Rab18/MAPK/FosB2 signaling axis. (A) Representative immunofluorescent (IF) images of indicated MDA-MB-231 cells with DAPI and phalloidin staining. Where indicated, cells were treated with 2.5 µg/mL 2′3′-cGAMP for 24 h. (B) Representative IF images of indicated HeLa cells. Where indicated, HeLa cells were treated with 2.5 µg/mL 2′3′-cGAMP alone, PD-0325901 (1 µM) alone, or in combination for 24 h. (C,D) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for either 18 h (C) or 36 h (D). Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (E) RT-PCR analysis of mRNA expression of indicated MMPs from MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP alone or in combination with SCH772984 (2 µM) for 12 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test). (F) RT-PCR analysis of mRNA expression of indicated MMPs from indicated MDA-MB-231 cells treated with 2.5 µg/mL 2′3′-cGAMP for 18 h. Error bars were calculated as the mean ± SD; n biological replicates are as indicated in each panel. * p < 0.05 (one-way ANOVA test).

    Article Snippet: The antibodies used in this study are listed as follows: anti-FOSB2 antibody (Cell Signaling Technology 2251S, Danvers, MA, USA); anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (Cell Signaling Technology 4370S); anti-ERK1 antibody (Santa Cruz Biotechnology sc-271269, Dallas, TX, USA); anti-Rab18 antibody (Santa Cruz Biotechnology sc-393169); anti-Vinculin antibody (Santa Cruz Biotechnology sc-25336); anti-GAPDH antibody (Proteintech 60004-1-Ig, Rosemont, IL, USA); anti-rabbit immunoglobulin G (IgG); horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology 7074); anti-mouse IgG; and HRP-linked antibody (Cell Signaling Technology 7076).

    Techniques: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing